OPTI-BIOME® Bacillus subtilis MB40 is durable even in harsh pH environments. An in vitro study was designed to simulate the inherent pH and retention time of a substance (meal) held in the human stomach.6 Percent survival of OPTI-BIOME® Bacillus subtilis MB40 was tested by counting viable spores after a 4 hour incubation at pH 2, 4, 6, and 7 at 37°C. This count was then divided by the total count of the unincubated control to generate the percent survival shown in Figure 2 below.

This ability to retain high levels of activity after exposure to extreme temperature ranges for prolonged periods of time (longer than 2 hrs) makes OPTI-BIOME® Bacillus subtilis MB40 an ideal candidate to be used(typically a 10-15 min process exposure)

OPTI-BIOME® Bacillus subtilis MB40 is very shelf stable. Stability testing of OPTI-BIOME® Bacillus subtilis MB40's stability was conducted over a 24-month period at ambient temperature and showed that OPTI-BIOME® Bacillus subtilis MB40 has at least a 24 month shelf life. OPTI-BIOME® Bacillus subtilis MB40 was counted at the time of manufacturing and stored at ambient temperature (21±30C.) Total spore count analyses were conducted at several intervals during the 24 month period. Figure 3 below shows OPTI-BIOME® Bacillus subtilis MB40's shelf stability over 24 months at ambient temperature.